The extracted bioactive metabolites using ethyl acetate were tested as antimicrobial, anticancer, antiviral and antioxidant agents. The potentiality of chloroform, hexane, petroleum ether and ethyl acetate for extraction of the active compounds was tested showing that ethyl acetate was the best. Consequently, Box-Behnken design was applied to estimate the optimized levels of each independent variable showing that the optimized conditions were malt extract, 3 (g/l) peptone, 0.75 (g/l) salinity, 50% pH, 4 culture age, 4 days inoculum size, 0.5 ml temperature 30☌ and incubation time 5 days, which caused an increase in the antimicrobial activity to 25 mm, which denotes an approximately 1.8 fold increase comparing with the pre-optimized conditions. The obtained results showed that malt extract, pH and temperature were the key factors affecting the antimicrobial activity.
Multi-factorial design in terms of Placket Burman design was implemented to predict the critical factors influencing the production of the antibacterial agents by A. hydrophila was studied showing the highest productivity after 5 days incubation. Time course production of the antibacterial agents by A. hydrophila was morphologically and genetically identified as Aspergillus terreus SHE05 and the corresponding sequence was recorded in the GenBank database with accession no. The most promising isolate giving the highest antibacterial activity (14 mm) against A. Antagonism effect against Aeromonas hydrophila on purpose and other pathogen was estimated.
Seven morphologically distinct marine fungi were isolated from sediment and Seawater samples at different sites along Alexandria seashore.
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